Complex sequence and “HapMap” shed light on maize’s “wonderful diversity” could help in future efforts to adapt the plant to a warming climate
Cold Spring Harbor, NY — A four-year, multi-institutional effort co-led by three 黑料吃瓜资源 (CSHL) scientists culminated today in publication of a landmark series of papers in the journal Science revealing in unprecedented detail the DNA sequence of maize (Zea mays). Maize, or corn, as it is commonly called by North American consumers, is one of the world鈥檚 most important plants and the most valuable agricultural crop grown in the United States, representing $47 billion in annual value.
The sequence spans 2.3 billion DNA base-pairs and contains some 32,500 genes, or about one-third more than the human genome, according to the team that assembled it over the last four years. This version of the maize genome鈥攖aken from a variant called B73鈥攊s important, in part, because it is regarded by the scientific and agricultural communities as a 鈥渞eference鈥� version. It represents a significant filling-in of gaps in a draft maize sequence announced a year and a half ago, but more importantly, comes with what amounts to a detailed reference manual, a set of comprehensive annotations.
A scientific and practical landmark
鈥淏oth the sequence itself and the annotations are a landmark,鈥� says Doreen Ware, Ph.D., a co-principal investigator of the project whose CSHL lab focused primarily on the annotation and evolutionary analysis. Principal investigator of the Maize Genome Project of the National Science Foundation, which provided funding with the U.S. Department of Agriculture (USDA) and the U.S. Department of Energy, is Richard Wilson, Ph.D., of Washington University, St. Louis. Other co-principal investigators include scientists from the University of Arizona and Iowa State University.
In a parallel effort, Ware鈥檚 CSHL team also helped generate the first so-called 鈥淗apMap鈥� of maize in collaboration with Edward Buckler, a USDA scientist. The HapMap, a shorthand for haplotype map, gauges diversity in the maize genome by comparing 27 distinct genetic lines of the plant with the reference version.聽A human HapMap, prepared in conjunction with the Human Genome Project, has revealed important linkages between genetic variations and risk for major diseases in ethnically and geographically distinct human populations.
鈥淲hat鈥檚 important about the maize project,鈥� says W. Richard McCombie, Ph.D., CSHL Professor, co-principal investigator on the maize genome project, and a pioneer in genome sequencing efforts, 鈥渋s that it provides a reference DNA sequence for the most important agricultural crop in the U.S., making it much easier for people to look at the many variants of different strains or 鈥榓ccessions鈥� of maize.鈥� New sequencing technologies, just now becoming commercially viable, will now 鈥渁nalyze other maize strains by comparing them to this one鈥攁lbeit at dramatically lower costs and accelerated speeds,鈥� McCombie notes.
Another of the CSHL co-project leaders, Professor Robert Martienssen, Ph.D., puts the maize sequencing project into historical perspective.聽Martienssen, a world leader in research on transposons鈥攂its of DNA that copy and insert themselves randomly across the chromosomes鈥攏oted that transposable elements are found in all organisms, 鈥渂ut were discovered in maize more than 60 years ago,鈥� by CSHL鈥檚 Barbara McClintock, who was honored with a Nobel Prize for the discovery in 1983. 鈥淚t is a remarkable achievement to now be able to visualize transposons in such detail in the maize genome sequence,鈥� Martienssen says.
鈥淲onderful diversity鈥� and its evolutionary implications
Transposons play a particularly dramatic role in the maize genome, as the sequence clearly shows.聽Nearly 85 percent of the genome is composed of hundreds of families of transposable elements, distributed unevenly across the 10 maize chromosomes.聽This is one aspect of the maize genome鈥檚 complexity; another is its variability between different 鈥渋ndividuals.鈥澛燤aize plants from two different strains are, on average, more genetically different than humans are different from chimpanzees.
鈥淭he wonderful diversity that we see in maize today is the product of many things,鈥� Ware explains.聽鈥淪everal million years ago, the maize genome effectively doubled in size, to 20 chromosomes, and then, subsequently, returned to its current size of 10 chromosomes.聽In the detailed sequence that we now have obtained, we can begin to study the impact of that 鈥榙oubling event.鈥欌��
Ware notes that genome doubling is not uncommon in the plant kingdom, and hypothesizes that 鈥渋t may be a very successful way for speciation to occur.鈥澛燨nce an organism can draw on two full sets of essentially the same genes, it can begin 鈥渢o de-evolve certain of the genes in one set and adapt them to some other function鈥攊mportantly, without compromising the gene鈥檚 original function,鈥� she says. In this way, hypothetically, the plant could become more 鈥渃apable,鈥� in genetic terms, over long periods of time.
The maize reference genome will also provide a basis for close investigation of the impact of human breeding and trait selection, in the much more proximate historical era since the plant鈥檚 domestication, some 10,000 years ago.聽Maize is known to have evolved from a common grass found in Mexico and Central America called teosinte.聽It was human intervention鈥攂reeding鈥攖hat led to full domestication and unimagined value and utility. As maize became ever more useful to people—as food and animal feed—it was carried beyond the volcanic soils of central Mexican valleys and the indigenous peoples of North and South America to the far reaches of the planet, at first by European mercantile and imperial powers of the sixteenth and seventeenth centuries. It has long been a central cultural element in the region of its initial domestication, but has since worked its way into the sinews of many other cultures. This week, for example, in schoolrooms across America, tales are being told about how 鈥淚ndian corn鈥� was served at the very first Thanksgiving meals in early-seventeenth-century New England.
The genome as a starting point for improving an indispensable crop
Today maize is an important, if controversial, source of biomass for a wide range of industrial applications, and, very recently, a prime source of biofuel. CSHL鈥檚 Ware, who is also a scientist at the USDA, has a keen interest in thinking about maize in terms of its identity as agricultural germplasm.聽鈥淲hat we鈥檙e trying to do is identify what is best鈥攁nd keep the best in the germplasm,鈥� she says. 鈥淭he 鈥榖est鈥� will vary, depending on what the environment is. What鈥檚 best in Missouri is not necessarily best in Washington state. That helps explain why having a HapMap of maize will be useful for breeders in producing improved corn plants.
鈥淲e鈥檙e trying to use the genome to understand not only the differences between individual lines, but also to identify what differences, in genetic terms, are still available within maize. Ideally, we鈥檇 like to understand the function of every gene. In comparing different lines, we want to find genes associated with what we call quantitative traits鈥攇enes that affect traits of importance to agriculture, everything from the size of the seeds to when the plant flowers to whether it can tolerate drought or dampness.
鈥淲ith climate change upon us, there is great need in the years ahead to adapt existing germplasm to future needs,鈥� Ware suggests. 鈥淲ill we be able to grow maize 20 years from now in the same places we do today? What will we need to do to improve this extremely valuable plant?鈥澛燱ith the reference version of the maize genome and tools like the maize HapMap now in the public domain, the search will proceed with a new intensity, made possible by a treasure trove of new data.
In addition, The DNA Learning Center of 黑料吃瓜资源 has just launched a series of podcasts and short videos explaining the scientific importance of the maize genome as well as the cultural and historical significance of the maize plant itself.聽These can be accessed at: For additional educational information about maize and genetics, please visit:
Written by: Peter Tarr, Senior Science Writer | [email protected] | 516-367-8455
Citation
The maize reference genome and the haplotype map are published online today ahead of print in the journal Science, in addition to a supplementary poster on the maize genome placing the plant and the sequencing project in historical and cultural perspective.聽In addition to CSHL鈥檚 Ware, the co-lead author of the reference genome paper is Patrick S. Schnable, Ph.D., of Iowa State University.聽 The paper is entitled, 鈥淭he B73 Maize Genome: Complexity, Diversity, and Dynamics.鈥澛� The corresponding author is Richard K. Wilson.
The Hap Map paper, appearing simultaneously online ahead of print in Science, is entitled, 鈥淎 First-Generation Haplotype Map of Maize;鈥� the corresponding author is Edward S. Buckler.
Doreen Ware and her CSHL colleagues have also played an important role in authoring聽 a series of papers probing some of the biology underlying the maize reference genome that are being published concurrently in the journal Public Library of Science (PLoS) Genetics.聽 Please visit: .