黑料吃瓜资源

Cold Spring Harbor, NY — For years, cancer experts have realized that cancerous cells behave in certain ways like stem cells, unspecialized cells that when exposed to certain signals, can 鈥渄ifferentiate.鈥�

When a stem cell differentiates, it starts down a one-way path that will result in its specialization and eventually its death. For instance, a stem cell in the breast can become a luminal cell, one of the breast鈥檚 鈥渕ilk factories.鈥� Such cells have a limited life span. Cancer cells resemble stem cells not because they can turn into other cell types, but because in developmental terms, they seem to go in the opposite direction: they begin to run through multiple layers of stop signs and barricades and just keep on multiplying.

Assistant Professor Camila dos Santos of 黑料吃瓜资源 (CSHL) is studying stem cells in the breast for clues about what changes occur when normal breast cells become cancerous. Today, a team led by dos Santos, in collaboration with Assistant Professor William Pomerantz at the University of Minnesota and Professor Gregory Hannon of Cancer Research UK, Cambridge Institute, identify a protein that they show must be present in order for mammary stem cells to perform their normal functions.

When the researchers genetically removed or chemically inhibited the protein, called BPTF, stem cells could no longer maintain their 鈥渞enewing鈥� state and began to take on the character of specialized breast cells—and then soon died.

鈥淭hat was very exciting for us,鈥� says dos Santos, 鈥渂ecause that鈥檚 exactly what we want to drive breast cancer cells to do. We want to take away their stem cell-like qualities—especially their ability to multiply indefinitely. We are testing the idea that a drug that inhibits BPTF might have the same effect in cancer cells as in stem cells—it could cause them to differentiate and then die.鈥�

When studying how normal cells change into cancer cells, dos Santos and other cancer researchers pay close attention to gene expression. Every cell in the breast, including stem cells, contains the full human genome. One way of thinking about what differentiates a breast cell from a heart cell is that each cell type expresses different subsets of genes.

The same is also true within each organ. In the breast, the ducts designed to carry milk during and after pregnancy are composed of two highly specialized cell types and a niche of stem cells that gives rise to both types. Each of these different cell types expresses different groups of genes at different times over the lifespan of an individual.

As the illustration below indicates, the hollow 鈥渢ube鈥� that forms the milk duct is built from luminal cells; these are surrounded by a thin layer of cells called myoepithelial cells. Receptors on the surface of the myoepithelial cells are designed to interact with a hormone, oxytocin, released during lactation. This interaction causes the myoepithelial cells—on the outer layer of the ductal structure—to contract, squeezing the luminal cells within. Those luminal cells are the breast鈥檚 milk factories.

BPTF鈥檚 epigenetic role in exposing and hiding genes

BPTF, identified by dos Santos and colleagues as essential for mammary stem cell maintenance, is a protein with a very specialized function. It is what biologists call a chromatin remodeling factor. Chromatin is the packaging that enables six linear feet of DNA in each of our cells to be compressed inside the microscopic nucleus.

With so much DNA squished into such a small space, it stands to reason that expressing a gene in the 鈥渕iddle鈥� of the bundle might require loosening the packing material to expose that segment of DNA to the machinery that copies it into an RNA molecule. This copying is the first step in using the gene鈥檚 鈥渂lueprint鈥� to manufacture a needed protein. Chemical modifications to chromatin鈥攁nd even more specifically, to the histone proteins that provide 鈥渟pools鈥� around which the DNA is wound鈥攁re called epigenetic modifications.

鈥淚t has become very clear that the opening up or tightening of chromatin, to expose or hide genes in our chromosomes, plays a role in cancer progression,鈥� dos Santos says. 鈥淔or instance, exposing a gene at a particular moment might help a cancer cell bypass a 鈥榮top sign鈥� in a growth pathway.鈥�

The research published today shows that BPTF is part of a regulatory system that opens chromatin and changes gene expression, specifically in mammary stem cells. This opening of the chromatin turns out to be critical in the ability of the stem cell to remain 鈥渋mmortal鈥�—to give rise to daughter stem cells that will also help maintain a tissue such as the breast, and seeding it, at different times of life, with specialized cells. For example, during puberty, when the breast develops, or during pregnancy, when the breast gears up to produce milk.

鈥淲e now know that mammary stem cells are highly dependent on BPTF. The next task is to explore if can we use that dependency to target stem cell-like programs in breast cancer cells,鈥� dos Santos says.

Written by: Peter Tarr, Senior Science Writer | [email protected] | 516-367-8455

Funding

The research discussed here was supported by CSHL Cancer Center Support Grants 5P30CA045508 and 5P30CA045508-28; P01 award #2P01CA013106; the Sidney Kimmel Cancer Foundation; the Pershing Square Innovator Award; the Rita Allen Scholar Award; the V Foundation Scholar Award; and the Manhasset Coalition Against Breast Cancer Award.

Citation

鈥淏PTF maintains chromatin accessibility and the self-renewal capacity of mammary gland stem cells鈥� appears online June 1, 2017 in Stem Cell Reports. The authors are: Wesley D. Frey Anisha Chaudhry Priscila F. Slepicka, Adam M. Ouellette, Steven E. Kirberger, William C. K. Pomerantz, Gregory J. Hannon and Camila O. dos Santos. The paper can be accessed at: