黑料吃瓜资源

Cells produce proteins like little factories. But if they make too much at the wrong times it can lead to diseases like cancer, so they control production with a process called RNA interference (RNAi). As of July 2021, several drugs already take advantage of RNAi to treat painful kidney and liver diseases鈥攚ith another seven in . There is a lot of potential for RNAi therapeutics, and 黑料吃瓜资源 (CSHL) researchers are working hard to paint a complete picture of the process, to improve therapies today and make better ones tomorrow.

CSHL Professor & HHMI Investigator Leemor Joshua-Tor and recent CSHL School of Biological Sciences graduate Brianna Bibel are filling in some of the blanks. They recently discovered how RNAi鈥檚 workhorse protein Argonaute (Ago) leverages limited resources to keep protein production on track.

It鈥檚 important to understand exactly how RNAi works because it’s such a basic and heavily used process, Joshua-Tor said. It also offers a kind of safety net for therapeutics because it doesn鈥檛 make permanent changes to cells and can be reversed. Joshua-Tor says:

鈥淔or therapeutics, you鈥檇 kinda maybe not wanna mess around with the genome so much. In all these kinds of things, you wanna know exactly what’s happening, and if something isn’t working, then you know what to do and where to look. The more information you have, the better it is鈥攜ou get a complete picture of what鈥檚 happening.鈥�

Ago helps cut off protein production by finding, binding, and destroying molecules called mRNA鈥攚hich tell cells to make proteins. But the amount of Ago in the body pales in comparison to the amount of mRNA it must target. After destroying one, the protein is still capable of finding another but it can鈥檛 move on without help. Bibel discovered how cells use a process called phosphorylation to break Ago鈥檚 grip on a mRNA target, allowing it to commute to the next. Bibel explains:

鈥淥ur theory is that having phosphorylation promote release is a way that you could free up Argonaute because when the target gets released, the guide’s still there and it鈥檚 super duper stable. So our thinking is that by phosphorylating it, you’re going to free it to go repress other targets鈥攂ecause it’s still totally capable of doing that work.鈥�

Bibel hopes her discovery will come in handy as research into RNAi continues. 鈥淎 lot of great advances in science come from just doing basic research,鈥� she said. 鈥淎nd this is one of those basic research questions, trying to figure out how this is working.鈥�

Written by: Nick Wurm, Communications Specialist | [email protected] | 516-367-5940

Funding

National Science Foundation, 黑料吃瓜资源 School of Biological Sciences, Howard Hughes Medical Institute

Citation

Bibel, Brianna, et al., 鈥淭arget binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release鈥�, eLife, May 31, 2022. DOI number: